![Detection of α-GalCer (d18:0) species with several lengths of side chains in bile.(A) HRMS chromatograms of HexCer detected in bovine bile by SFC/HRMS/MS. The red shadow indicates the peaks coincident with the theoretical RTs of α-GalCer. The precursor ions as [M+H]+ are m/z 674.5565, 688.5722, 702.5878, 716.6035, 730.6191, and 800.6974, respectively. The HRMS chromatograms were plotted from the theoretical m/z ± 7 ppm of candidate HexCer molecular species. (B) The concentrations of α-GalCer, β-GalCer, and β-GlcCer of indicated species were detected in bovine bile. (C) The ratio of α-GalCer to β-GalCer quantities in the indicated GalCer species was detected in bovine bile by SFC/HRMS/MS.](https://cdn.rupress.org/rup/content_public/journal/jem/222/2/10.1084_jem.20240728/1/jem_20240728_fig5.png?Expires=1767647719&Signature=wzRWcTIfCyeryqsVWCPSu7dM1TO9RG2lpEYvOtlagj0tDZQPsHbqz5twkTClJoMnoZAXeaB5lQbC0Cuar6FiclHQcga0rx91NCVtTaM4mMkayZ7S-fnAZYqQkmfxbATxrpVQ71ksNk3fRLGjpM0-6PHLBrC1vAIG8oB7YCimFE9rNj74rJc3lAVXfLflroBVPcIirgu6qugGi5Fy6snjz4bGc7dGWddGw9gPjqPSuZNaAhDaDGmWH3Dl2UinWlxfcu2LAhj46Z~vvDZArJT7PS1j-6r9RMeFPmH-3MwUC-cpG2oq5UGYBgwJWSRKLQx-WMNGbJHn1Y9FAp7sACdptA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Figure 5.
Detection of α-GalCer (d18:0) species with several lengths of side chains in bile. (A) HRMS chromatograms of HexCer detected in bovine bile by SFC/HRMS/MS. The red shadow indicates the peaks coincident with the theoretical RTs of α-GalCer. The precursor ions as [M+H]+ are m/z 674.5565, 688.5722, 702.5878, 716.6035, 730.6191, and 800.6974, respectively. The HRMS chromatograms were plotted from the theoretical m/z ± 7 ppm of candidate HexCer molecular species. (B) The concentrations of α-GalCer, β-GalCer, and β-GlcCer of indicated species were detected in bovine bile. (C) The ratio of α-GalCer to β-GalCer quantities in the indicated GalCer species was detected in bovine bile by SFC/HRMS/MS.