ATP release and dye uptake is supported more effectively by heptameric versus hexameric hPANX1 concatemers. hPANX1-deleted HEK293T cells were transfected with hPANX1(TEV) or hexameric and heptameric hPANX1 concatemers along with either TEVp or empty vector and maintained in Trovan (25 µM) following transfection to block hPANX1 channel activity. (A and B) The cells were washed and incubated in Trovan or vehicle (DMSO) before assessing ATP release by luciferase assay (A) or TO-PRO-3 dye uptake by flow cytometry (B). The TEVp-activated ATP release and dye uptake that was Trovan-dependent (DMSO-Trovan) was considered to be hPANX1-mediated. Analyses by two-way ANOVA with Šídák’s multiple comparisons test.