Heptameric concatemers closely match unitary properties of hPANX1 channels in hPANX1-deleted HEK293T cells. (A) Inside-out patch recordings of TEVp-activated channels from hPANX1-deleted HEK293T cells expressing hPANX1(TEV) channels at the indicated membrane potentials. (B) Overlay of i-V relationships for TEVp-activated hPANX1(TEV) channels and hexameric and heptameric hPANX1 concatemers, with linear regressions fitted at positive and negative potentials (shaded regions is the 95% confidence interval; data from Fig. 3, Fig. 4, and Fig. S2). (C) Unitary conductance estimates from individual patches expressing the indicated construct; values from hPANX1 and the heptamer were significantly greater than those from the hexamer. Analysis by two-way ANOVA with Šídák’s multiple comparisons test. (D) Overlay of open time distributions from the indicated constructs recorded at +80 mV, with their corresponding Gaussian fits and channel mean open times. (Data from Fig. 3, Fig. 4, and Fig. S2). (E) Channel mean open times for hPANX1(TEV) and the hexameric and heptameric concatemers at the indicated potentials. Analysis by two-way ANOVA with Tukey’s multiple comparisons test.