Unitary channel properties of heptameric hPANX1 concatemers in hPANX1-deleted HEK293T cells. (A) Inside-out patch recordings of TEVp-activated CBX-sensitive channels from hPANX1-deleted HEK293T cells expressing concatenated heptameric hPANX1 channels. (B) Recordings of TEVp-activated channels at the indicated membrane potentials. (C) Single channel i-V relationship for heptameric hPANX1 concatemers, with estimated conductance from the fitted linear regression at positive and negative potentials (shaded regions is the 95% confidence interval). (D) Channel PO at the indicated membrane potentials. (E) Open time distribution fitted with a Gaussian function to estimate channel mean open time. Insets: Expanded channel records at a faster time base (from region indicated in B). (F) Channel mean open times at the indicated membrane potentials. Statistical analyses by ANOVA with Tukey’s multiple comparisons test.