In hPANX1-deleted HEK293T cells, receptor-activated currents are evoked in hexameric and heptameric concatemers with intact intersubunit linkers. (A and C) Whole-cell recordings of PE-activated, CBX-sensitive currents in α1DAR-expressing hPANX1-deleted HEK293T cells cotransfected with concatenated hexameric (A) and heptameric (C) hPANX1 channels; exemplar ramp I-V curves and time series of peak current at +80 mV (insets). (B and D) Quantification of the peak CBX-sensitive current density (pA/pF) from hexameric (B) and heptameric (D) hPANX1 concatemers under the indicated conditions. Statistical analysis by ANOVA with Tukey’s multiple comparisons test. Note that PANX1-like currents developed even when the intersubunit linkers were intact (i.e., when the pipette did not contain TEVp).
Figure 2.

In hPANX1-deleted HEK293T cells, receptor-activated currents are evoked in hexameric and heptameric concatemers with intact intersubunit linkers. (A and C) Whole-cell recordings of PE-activated, CBX-sensitive currents in α1DAR-expressing hPANX1-deleted HEK293T cells cotransfected with concatenated hexameric (A) and heptameric (C) hPANX1 channels; exemplar ramp I-V curves and time series of peak current at +80 mV (insets). (B and D) Quantification of the peak CBX-sensitive current density (pA/pF) from hexameric (B) and heptameric (D) hPANX1 concatemers under the indicated conditions. Statistical analysis by ANOVA with Tukey’s multiple comparisons test. Note that PANX1-like currents developed even when the intersubunit linkers were intact (i.e., when the pipette did not contain TEVp).

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal