Hexameric and heptameric concatemers generate comparable whole cell currents in control cells. (A and B) Schematic of configuration for whole-cell recording of Cas9/GFP-expressing HEK293T cells with TEVp in the pipette to cleave subunit linkers of concatenated hexameric (A) and heptameric (B) PANX1 channels; exemplar ramp I-V curves and time series of peak current at +80 mV (insets) from the indicated constructs and conditions. Note that CBX-sensitive hPANX1-like currents developed only in cells expressing 6(0CT) and 7(0CT) constructs with truncated C-tails, and only when the pipette contained TEVp to cut the intersubunit linkers.