Hexameric and heptameric concatemers generate comparable whole-cell currents in control and hPANX1-deleted HEK293T cells. (A and B) Schematic of configuration for whole-cell recording of hPANX1-deleted HEK293T cells with TEVp in the pipette to cleave linkers of concatenated hexameric (A) and heptameric (B) hPANX1 channels; exemplar ramp I-V curves and time series of peak current at +80 mV (insets) from the indicated constructs and conditions. Note that CBX-sensitive hPANX1-like currents developed only in cells expressing the 6(0CT) and 7(0CT) constructs with truncated C-tails, and only when the pipette contained TEVp to cut the inter-subunit linkers. (C and D) Quantification of the peak CBX-sensitive current density (pA/pF) from hexameric (C) and heptameric (D) hPANX1 concatemers under the indicated conditions. Statistical analysis by three-way ANOVA with Šídák’s multiple comparisons test. (E) Comparison of current density from hexameric and heptameric in either control (Cas9/GFP) or hPANX1-deleted HEK293T cells. Statistical analysis by two-way ANOVA with Šídák’s multiple comparisons test.