Schematic of hPANX1 concatemer design. (A) Illustration of hexameric and heptameric hPANX1 concatemers with intact C-tails. An expanded view of the final two subunits shows the retained C-terminal caspase site and the linker, containing a Flag epitope sequence and a site for TEVp-mediated cleavage; a C-terminal GFP is appended to the last subunit in the concatemers. (B and C) Schematized views of final two subunits and expected conformations of 6(6CT) and 7(7CT) hPANX1 concatemers with intact C-tails (B) and of 6(0CT) and 7(0CT) concatemers with C-tails truncated at the C-terminal caspase site (C).