SG group 1 ILC dynamics during MCMV infection. (A) Flow cytometric quantification of total CD45⁺ leukocytes in MCMV-infected SGs over time shown as ratio to naïve mice. (B) Representative flow cytometry plots of SG leukocytes in naïve mice and at 8 dpi. (C) Quantification of group 1 ILC subsets at 50 dpi. (D) Flow cytometry plots of characteristic surface markers among SG group 1 ILC subsets (indicated by color) in naïve or infected (10 dpi) mice. (E) Quantification of indicated immune cell populations in the spleen at 16 dpi. gdT, gamma delta T cells. (F and G) Representative histograms (F) and quantification (G) of IFN-γ⁺ T cells and group 1 ILCs in the SG at 10 dpi. (H) Representative flow cytometry plots and quantification of IFN-γ⁺ SG group 1 ILCs at 10 dpi. (I) Quantification of GzmB⁺ CD8 T cells in the SG at 16 dpi. (J and K) Viral M86 gDNA levels in blood and spleens of WT and Ncr1^CreTgfb1^fl mice at 4, 7, 10, or 16 dpi, measured by qPCR. n.d., not detectable. (L) Viral M86 gDNA levels in SGs of WT, Ncr1^CreTgfb1^fl, and Ncr1^CreTgfbr2^fl mice at 16 dpi. (A and B) Data pooled from two independent experiments with total n = 6 mice per timepoint, or (C–I) representative of two to three independent experiments with n = 4–10 mice per group, or (J–L) pooled from two independent experiments with total n = 5–8 mice per condition. Error bars display means ± SD. Statistical significance was calculated using one-way ANOVA or two-tailed t test; *P < 0.05 and ****P < 0.0001. ns, not significant.