Characterization of the AID-v2 library. (a) Comparison of normalized colony sizes in the AID-v1 and AID-v2 libraries grown according to Fig. 2 c and Fig. 4 a, respectively. ρ, spearman correlation coefficient. (b and c) Normalized colony sizes in the AID-v2 library grown according to Fig. 4 a on galactose medium with or without 1 μM 5-Ph-IAA. c, essential and non-essential ORFs are marked; top and right, normalized frequency distributions of normalized colony sizes in each category. (d and e) Frequency of 5-Ph-IAA–dependent fitness defects in the AID-v2 library according to the extent of OsTIR1-dependent degradation phenotypes in the AID-v1 library (d) or according to protein localization, location of the C-terminus (cytosol – in, lumen of organelles or extracellular – out) or protein abundance estimates from the AID1-v1 library (e).