Fitness defects in the AID libraries upon induction of protein degradation. (a and b) Normalized colony sizes in the AID-v1 library grown according to Fig. 2 c. (b) Essential and non-essential ORFs are marked; top and right, normalized frequency distributions of colony sizes in each category. (c and d) Frequency of OsTIR1-dependent fitness defects in the AID-v1 library according to the extent of OsTIR1-dependent degradation phenotypes (c) or according to localization, location of the C-terminus (cytosol – in, lumen of organelles or extracellular – out) or abundance of the mNG-AID*-3myc-tagged proteins (d).