A Cdc48 complex member displays CDC characteristics. (A) Left: Brightfield microscopy images of Cdc48, before and after induction of the degron system for 24 h. A distinct and synchronized altered morphology can be observed after protein depletion as previously shown. Scale bar: 5 µm. Center: Growth curves of Cdc48, with induction of the degron (gray curve) or not (green curve). Lethality is seen upon depletion of Cdc48. An average of three repeats with their standard deviation is shown. Right: Flow cytometry measurements for Cdc48, with or without 4 h induction. Without induction (green), a typical histogram for cycling cells is appreciated, while an arrest in G2/M stage can be seen upon protein depletion (gray and upper right corner schematic). (B) Same as panel A, but for Npl4, a Cdc48 interactor. The growth curve is identical to the one displayed in Fig. 3 C.