The C′AID-GFP library facilitates the study of CDC genes. (A) Brightfield microscopy images of strains previously assigned as CDCs, after induction of the degron system for 24 h. A small schematic, recreating the original cartoons (Hartwell et al., 1973) is depicted in the lower left corner of each image. Scale bar: 5 µm. (B) Brightfield microscopy images of strains here suggested as CDCs, before and after induction of the degron system for 24 h. In every case, a distinct and synchronized altered morphology can be observed after protein depletion. Scale bar: 5 µm. (C) Growth curves for strains in panel B, with induction of the degron (orange curve) or not (green curve). Growth defects or lethality are seen upon depletion of the indicated tagged protein. An average of three repeats with their standard deviation is shown. (D) Flow cytometry measurements for strains in panel B, with or without a 3 h induction. Without induction (green), a typical histogram for cycling cells is appreciated in each case, while different phenotypes point to issues in the cell division cycle in each protein-depleted case (orange). A schematic of the cell cycle (upper right corner of each panel) shows which phase is affected (orange blocks and arrows).