New mitochondrial morphology and distribution phenotypes appear after protein depletion. (A) Fluorescent images of strains before and after induction of the degron system for 24 h, for previously undescribed proteins required for correct mitochondria morphology and distribution. After depletion of the tagged proteins (green), the mitochondria look aberrant (magenta). (B) Bar graph showing the oxygen consumption rate derived from respiration for control and selected strains, before and after 7 h of induction with 5-Ph-IAA. Significant changes for each group with the control and for each induced versus their corresponding uninduced group are marked (one-way ANOVA, FDR 0.05). (C) Scatter plot showing the MitoTracker signal intensity per cell after 24 h of induction versus strain rank. Many proteins belonging to the ergosterol biosynthesis pathway (stars) display elevated mitochondrial signal intensity (which could either be changes in membrane potential or in shape) compared to the median. (D) Fluorescent images of selected strains whose depleted protein participates in the ergosterol biosynthesis pathway, without or with 4 h of induction. The mitochondrial morphology changes and the MitoTracker signal intensity (quantification of mean MitoTracker signal per cell ± SD in the MitoTracker images) increases (magenta) upon protein depletion (green). For all images, the scale bar is 5 µm.