The C′ AID-GFP library extends the degron approach to a proteome level. (A) Growth curves of control (WT) strain (blue, BY4741), the same strain transformed with the OsTIR1(F74G) and resistance to nourseothricin (NAT, green), or WT transformed only with the resistance to NAT (orange); all with 5 µM 5-Ph-IAA in DMSO (dark colors) or DMSO only (light colors). The strain containing the OsTIR1(F74G) presents a small growth defect when compared to WT or to the strain with matching resistance. (B) Bar graph displaying the duplication time calculated from the result from panel A. From a two-way ANOVA, the strain expressing the OsTIR1(F74G) grew at a significantly slower rate than the WT strain (P value 0.03, difference 8.3 ± 0.1%). Treatment with 5-Ph-IAA did not produce any effect. (C) Pie chart displaying the rate of survival of strains following the SWATting procedure. Colony presence was measured for the parental (C′ SWAT library) and derived (C′ AID-GFP) libraries, to calculate the 92% survival rate. (D) Pie chart displaying the fluorescent status of the top 500 most expressed proteins in the cell (Nash et al., 2020). For such abundant proteins a fluorescent signal was expected, therefore the estimation of 94% SWATting efficiency corresponds to the strains displaying a fluorescence signal. (E) Pie chart displaying the sequencing coverage (sequences from the whole library recovered by Anchor-Seq method), which was 98%. (F) Pie chart displaying the proportion of functional tags detected. Of the 5,084 strains detected by pooled sequencing, only 21 included variants that could compromise the function of the AID tag.