Conserved positive charge of kinesin-5 N-terminus. (A) Sequences of N-terminus of multiple kinesin-5 motors through the start of the catalytic motor domain core. KIF5B (kinesin-1) is included for comparison. Positive residues are blue. Bolded black residues indicate Cdk1 phosphorylation sites. Sc: Saccharomyces cerevisiae; Sp: Schizosaccharomyces pombe; Ce: Caenorhabditis elegans; Dm: Drosophila melanogaster; Xl: Xenopus laevis; Mm: Mus musculus; Hs: Homo sapiens. (B) Computational isoelectric point of N-terminus for kinesin motors in A. Expressed as most predictive model (average of all models).