Figure S3.
Pre-anaphase Kip1 spindle localization and full Cin8 anaphase quantification. (A) Quantification of background-subtracted Kip1-mNeonGreen pre-anaphase spindle fluorescence in wild-type TUB2 or tub2 mutant cells. Values are normalized to the median wild-type value. Bolded, outlined points represent the median for each replicate. Error bars are mean ± 95% CI for the replicate medians. TUB2: n = 105 cells; tub2-Δ430: n = 111 cells, P = 0.01; tub2-Δ438: n = 120 cells, P = 0.87; tub2-Δ438Q: n = 120 cells, P = 0.01. Statistics are Student’s t test with Welch’s correction compared with wild-type TUB2. * indicates P < 0.05. (B–E) Quantification of the proportion of Cin8-3GFP fluorescence along anaphase spindles in cells expressing (B) TUB2, (C) tub2-Δ430, (D) tub2-Δ438Q, or (E) tub2-Δ438Q from Fig. 3, E–H. Each quintile represents 20% of the total spindle length. Error bars are median ± 95% CI for all cells.

Pre-anaphase Kip1 spindle localization and full Cin8 anaphase quantification. (A) Quantification of background-subtracted Kip1-mNeonGreen pre-anaphase spindle fluorescence in wild-type TUB2 or tub2 mutant cells. Values are normalized to the median wild-type value. Bolded, outlined points represent the median for each replicate. Error bars are mean ± 95% CI for the replicate medians. TUB2: n = 105 cells; tub2-Δ430: n = 111 cells, P = 0.01; tub2-Δ438: n = 120 cells, P = 0.87; tub2-Δ438Q: n = 120 cells, P = 0.01. Statistics are Student’s t test with Welch’s correction compared with wild-type TUB2. * indicates P < 0.05. (B–E) Quantification of the proportion of Cin8-3GFP fluorescence along anaphase spindles in cells expressing (B) TUB2, (C) tub2-Δ430, (D) tub2-Δ438Q, or (E) tub2-Δ438Q from Fig. 3, E–H. Each quintile represents 20% of the total spindle length. Error bars are median ± 95% CI for all cells.

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