Figure S2.
Proportion of kinesin-5 close to spindle poles throughout anaphase. (A) Additional example montages of cells expressing Cin8-3GFP (green) and Spc110-tdTomato (magenta), similar to Fig. 2 C. The first time point is 10 min prior to anaphase onset. Time interval = 2 min. Scale bar = 1 µm. (B) Quantification of the proportion of Cin8-3GFP spindle fluorescence intensity within 530 nm of both poles (left axis) and spindle length (right axis) as a function of time since anaphase onset. Error bars are mean ± 95% CI. n = 20 cells. (C) Additional example montages of cells expressing Kip1-mNeonGreen (green) and Spc110-tdTomato (magenta), similar to Fig. 2 E. The first time point is 10 min prior to anaphase onset. Time interval = 2 min. Scale bar = 1 µm. (D) Quantification of the proportion of Kip1-mNeonGreen spindle fluorescence intensity within 530 nm of both poles (left axis) and spindle length (right axis) as a function of time since anaphase onset. Error bars are mean ± 95% CI. n = 24 cells.

Proportion of kinesin-5 close to spindle poles throughout anaphase. (A) Additional example montages of cells expressing Cin8-3GFP (green) and Spc110-tdTomato (magenta), similar to Fig. 2 C. The first time point is 10 min prior to anaphase onset. Time interval = 2 min. Scale bar = 1 µm. (B) Quantification of the proportion of Cin8-3GFP spindle fluorescence intensity within 530 nm of both poles (left axis) and spindle length (right axis) as a function of time since anaphase onset. Error bars are mean ± 95% CI. n = 20 cells. (C) Additional example montages of cells expressing Kip1-mNeonGreen (green) and Spc110-tdTomato (magenta), similar to Fig. 2 E. The first time point is 10 min prior to anaphase onset. Time interval = 2 min. Scale bar = 1 µm. (D) Quantification of the proportion of Kip1-mNeonGreen spindle fluorescence intensity within 530 nm of both poles (left axis) and spindle length (right axis) as a function of time since anaphase onset. Error bars are mean ± 95% CI. n = 24 cells.

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