Figure S1.
Quantification of β-CTT effect on Bik1 and whole-cell example images. (A) Quantification of Bik1-3GFP spindle localization in the presence or absence of the β-CTT. TUB2 n = 87 cells, tub2-Δ430 n = 93 cells, P = 0.23. Values are normalized to the median wild-type value. Bolded, outline points represent the median for each replicate. Error bars are the mean ± 95% CI for the replicate medians. Statistics are Student’s t test for the replicate medians. (B) Example images from Fig. 1 showing the entire cell, including Bik1 quantified in A. The spindle poles are labeled with Spc110-tdTomato. Scale bars = 1 µm. (C) Quantification of Cin8-3GFP whole-cell fluorescence intensity. TUB2 n = 100 cells, tub2-Δ430 n = 111 cells. Values are normalized to the median wild-type value. Bolded, outline points represent the median for each replicate. Error bars are the mean ± 95% CI for the replicate medians. (D) Quantification of Kip1-mNeonGreen whole-cell fluorescence intensity. TUB2 n = 94 cells, tub2-Δ430 n = 98 cells, P = 0.0011. Values are normalized to the median wild-type value. Bolded, outline points represent the median for each replicate. Error bars are the mean ± 95% CI for the replicate medians. Statistics are Student’s t test for the replicate medians. * indicates P < 0.05; ** indicates P < 0.01.

Quantification of β-CTT effect on Bik1 and whole-cell example images. (A) Quantification of Bik1-3GFP spindle localization in the presence or absence of the β-CTT. TUB2 n = 87 cells, tub2-Δ430 n = 93 cells, P = 0.23. Values are normalized to the median wild-type value. Bolded, outline points represent the median for each replicate. Error bars are the mean ± 95% CI for the replicate medians. Statistics are Student’s t test for the replicate medians. (B) Example images from Fig. 1 showing the entire cell, including Bik1 quantified in A. The spindle poles are labeled with Spc110-tdTomato. Scale bars = 1 µm. (C) Quantification of Cin8-3GFP whole-cell fluorescence intensity. TUB2 n = 100 cells, tub2-Δ430 n = 111 cells. Values are normalized to the median wild-type value. Bolded, outline points represent the median for each replicate. Error bars are the mean ± 95% CI for the replicate medians. (D) Quantification of Kip1-mNeonGreen whole-cell fluorescence intensity. TUB2 n = 94 cells, tub2-Δ430 n = 98 cells, P = 0.0011. Values are normalized to the median wild-type value. Bolded, outline points represent the median for each replicate. Error bars are the mean ± 95% CI for the replicate medians. Statistics are Student’s t test for the replicate medians. * indicates P < 0.05; ** indicates P < 0.01.

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