Phosphorylation of SYP-5/6 C-termini mediate interactions between the SC central region and meiotic HORMA domain proteins. (A) Immunofluorescence images of pachytene nuclei from indicated genotypes lacking meiotic kleisin subunits (rec-8; coh-3 coh-4). DNA (blue), 3xFlag::SYP-5 (magenta), HIM-3 (green), and HTP-3 staining are shown. Scale bar, 5 µm. (B) Composite immunofluorescence images of early pachytene nuclei dissected from indicated genotypes showing SYP-5 (green) and HTP-3 (magenta) staining. Scale bar, 10 µm.