Figure S5.
Characterization of SC disassembly and SYP-5 phosphorylation in syp-5W543Asyp-6W687Amutants. (A) Immunofluorescence images of diplotene nuclei from wild type and syp-5W543Asyp-6W687A mutant animals stained for 3xFlag::SYP-5 (green) and HTP-3 (magenta). Scale bar, 25 µm. (B) Immunofluorescence images of pachytene nuclei from wild type and syp-5W543Asyp-6W687A animals showing DNA, 3Flag::SYP-5 (magenta), and SYP-5 pS541 (green) staining. Scale bar, 5 µm. (C) Western blot of worm lysates from the indicated genotypes probed against the Flag tag. HIM-3 was used as a loading control. (D) Graph showing the quantification of 3xFlag::SYP-5 signals normalized to the loading control, HIM-3. Changes in the normalized protein expression were analyzed by unpaired t test (n = 3). Source data are available for this figure: SourceData FS5.

Characterization of SC disassembly and SYP-5 phosphorylation in syp-5 W543A syp-6 W687A mutants. (A) Immunofluorescence images of diplotene nuclei from wild type and syp-5W543Asyp-6W687A mutant animals stained for 3xFlag::SYP-5 (green) and HTP-3 (magenta). Scale bar, 25 µm. (B) Immunofluorescence images of pachytene nuclei from wild type and syp-5W543Asyp-6W687A animals showing DNA, 3Flag::SYP-5 (magenta), and SYP-5 pS541 (green) staining. Scale bar, 5 µm. (C) Western blot of worm lysates from the indicated genotypes probed against the Flag tag. HIM-3 was used as a loading control. (D) Graph showing the quantification of 3xFlag::SYP-5 signals normalized to the loading control, HIM-3. Changes in the normalized protein expression were analyzed by unpaired t test (n = 3). Source data are available for this figure: SourceData FS5.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal