Conserved tryptophan residues in SYP-5/6 C-termini are essential for proper SC assembly. (A and B) Electrostatic potential of the C-terminal tails of SYP-5 (aa 536–547) (A) and SYP-6 (aa 682–691) (B) using AlphaFold models (AF-Q9N3E8-F1 and AF-A0A7R9SUK8-F1). Conserved tryptophan residues (SYP-5 W543 and SYP-6 W687) are labeled in green. (C) Composite immunofluorescence images of dissected gonads from wild type, syp-5W543Asyp-6W687A, and syp-5S541A W543Asyp-6T685A W687A animals showing HIM-3 (magenta) and 3xFlag::SYP-5 (green). Scale bar, 50 µm. (D and E) Graphs showing the percent viable and male self-progeny from wild type (n = 1,921), syp-5S541Asyp-6T685A (n = 1,526), syp-5W543Asyp-6W687A (n = 1,962), and syp-5S541A, W543Asyp-6T685A, W687A hermaphrodites (n = 1,156). ***, P < 0.001; ****, P < 0.0001 by unpaired t test.
Figure 5.

Conserved tryptophan residues in SYP-5/6 C-termini are essential for proper SC assembly. (A and B) Electrostatic potential of the C-terminal tails of SYP-5 (aa 536–547) (A) and SYP-6 (aa 682–691) (B) using AlphaFold models (AF-Q9N3E8-F1 and AF-A0A7R9SUK8-F1). Conserved tryptophan residues (SYP-5 W543 and SYP-6 W687) are labeled in green. (C) Composite immunofluorescence images of dissected gonads from wild type, syp-5W543Asyp-6W687A, and syp-5S541A W543Asyp-6T685A W687A animals showing HIM-3 (magenta) and 3xFlag::SYP-5 (green). Scale bar, 50 µm. (D and E) Graphs showing the percent viable and male self-progeny from wild type (n = 1,921), syp-5S541Asyp-6T685A (n = 1,526), syp-5W543Asyp-6W687A (n = 1,962), and syp-5S541A, W543Asyp-6T685A, W687A hermaphrodites (n = 1,156). ***, P < 0.001; ****, P < 0.0001 by unpaired t test.

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