Phosphorylation of SYP-5/6 is required for proper PLK-2 localization and chromosome remodeling in late meiotic prophase. (A) Composite immunofluorescence images of wild type and syp-5S541Asyp-6T685A animals showing DNA (blue) and PLK-2::mRuby (green) staining. Scale bar, 25 µm. Insets show zoomed-in views of the boxed regions with 3xFlag::SYP-5 (magenta) staining. Scale bar, 1 µm. (B) Immunofluorescence images of diakinesis nuclei from wild type and syp-5S541Asyp-6T685A animals showing HTP-3, 3xFlag::SYP-5 (green), and HTP-1/2 (magenta). Scale bar, 20 µm. Insets show representative bivalents from indicated genotypes showing HTP-3, HTP-1/2 (magenta), and SYP-5 (green) staining. Scale bar, 1 µm. (C) Immunofluorescence images of diakinesis nuclei from wild type and syp-5S541Asyp-6T685A animals showing DNA, histone H3 pT3 (green), and HTP-3 (magenta) staining. Scale bar, 5 µm. Insets show zoomed-in views of the boxed regions. Scale bar, 1 µm.
Figure 4.

Phosphorylation of SYP-5/6 is required for proper PLK-2 localization and chromosome remodeling in late meiotic prophase. (A) Composite immunofluorescence images of wild type and syp-5S541Asyp-6T685A animals showing DNA (blue) and PLK-2::mRuby (green) staining. Scale bar, 25 µm. Insets show zoomed-in views of the boxed regions with 3xFlag::SYP-5 (magenta) staining. Scale bar, 1 µm. (B) Immunofluorescence images of diakinesis nuclei from wild type and syp-5S541Asyp-6T685A animals showing HTP-3, 3xFlag::SYP-5 (green), and HTP-1/2 (magenta). Scale bar, 20 µm. Insets show representative bivalents from indicated genotypes showing HTP-3, HTP-1/2 (magenta), and SYP-5 (green) staining. Scale bar, 1 µm. (C) Immunofluorescence images of diakinesis nuclei from wild type and syp-5S541Asyp-6T685A animals showing DNA, histone H3 pT3 (green), and HTP-3 (magenta) staining. Scale bar, 5 µm. Insets show zoomed-in views of the boxed regions. Scale bar, 1 µm.

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