Additional characterization of SYP-5/6 phosphorylation and SC disassembly defects in syp-5/6 phosphomutants. (A) Immunofluorescence images of diplotene nuclei from wild type and syp-5S541Dsyp-6T685D mutant animals stained for HIM-3 (magenta) and SYP-5 (green). Scale bar, 25 µm. The inset shows the zoomed-in view of the boxed regions. Scale bar, 1 µm. (B) Immunofluorescence images of diplotene nuclei from cosa-1 and syp-5S541Dsyp-6T685D; cosa-1 mutants stained for HTP-3 (magenta) and SYP-5 (green). Scale bar, 25 µm. The inset shows the zoomed-in view of the boxed regions. Scale bar, 1 µm. (C) Immunofluorescence images of diplotene nuclei from cosa-1(tm3298) mutants. HTP-3 and SYP-5 pS541 staining are shown. Scale bar, 25 µm.
Figure S4.

Additional characterization of SYP-5/6 phosphorylation and SC disassembly defects in syp-5/6 phosphomutants. (A) Immunofluorescence images of diplotene nuclei from wild type and syp-5S541Dsyp-6T685D mutant animals stained for HIM-3 (magenta) and SYP-5 (green). Scale bar, 25 µm. The inset shows the zoomed-in view of the boxed regions. Scale bar, 1 µm. (B) Immunofluorescence images of diplotene nuclei from cosa-1 and syp-5S541Dsyp-6T685D; cosa-1 mutants stained for HTP-3 (magenta) and SYP-5 (green). Scale bar, 25 µm. The inset shows the zoomed-in view of the boxed regions. Scale bar, 1 µm. (C) Immunofluorescence images of diplotene nuclei from cosa-1(tm3298) mutants. HTP-3 and SYP-5 pS541 staining are shown. Scale bar, 25 µm.

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