Phosphorylation of SYP-5 S541 and SYP-6 T685 is critical for proper SC assembly and disassembly. (A) Composite immunofluorescence images of full-length gonads from wild type and syp-5S541Asyp-6T685A mutant animals stained for HIM-3 (magenta) and 3xFlag::SYP-5 (green). Scale bar, 50 µm. (B) Composite immunofluorescence images of late pachytene and diplotene region from wild type and syp-5S541Asyp-6T685A animals showing 3xFlag::SYP-5 (magenta) and GFP::COSA-1 (green) staining. Scale bar, 50 µm. Insets show zoomed-in views of the boxed regions with DNA staining (blue). Scale bar, 1 µm. (C) Zoomed-in views of the boxed regions in diplotene as indicated by colored boxes in B. DNA (blue), SYP-5 (magenta), and GFP::COSA-1 (green) staining are shown. Scale bar, 1 µm.
Figure 3.

Phosphorylation of SYP-5 S541 and SYP-6 T685 is critical for proper SC assembly and disassembly. (A) Composite immunofluorescence images of full-length gonads from wild type and syp-5S541Asyp-6T685A mutant animals stained for HIM-3 (magenta) and 3xFlag::SYP-5 (green). Scale bar, 50 µm. (B) Composite immunofluorescence images of late pachytene and diplotene region from wild type and syp-5S541Asyp-6T685A animals showing 3xFlag::SYP-5 (magenta) and GFP::COSA-1 (green) staining. Scale bar, 50 µm. Insets show zoomed-in views of the boxed regions with DNA staining (blue). Scale bar, 1 µm. (C) Zoomed-in views of the boxed regions in diplotene as indicated by colored boxes in B. DNA (blue), SYP-5 (magenta), and GFP::COSA-1 (green) staining are shown. Scale bar, 1 µm.

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