Purification and characterization of the SYP-5 pS541 antibody. (A) Immune serum against the SYP-5 phosphopeptide (pS541) was passed through affinity columns coupled to non-phospho (SYP-5 S541) and phosphopeptides (SYP-5 pS541). The unbound (flow-through) and bound (elution) fractions were used in dot blots of serially diluted peptides. (B) Immunofluorescence images of pachytene nuclei from wild type, syp-5S541A, and syp-6T685A mutants showing DNA, 3xFlag::SYP-5 or HA::SYP-6 (magenta), and SYP-5 pS541 (green) staining. Scale bar, 5 µm. Source data are available for this figure: SourceData FS1.
Figure S1.

Purification and characterization of the SYP-5 pS541 antibody. (A) Immune serum against the SYP-5 phosphopeptide (pS541) was passed through affinity columns coupled to non-phospho (SYP-5 S541) and phosphopeptides (SYP-5 pS541). The unbound (flow-through) and bound (elution) fractions were used in dot blots of serially diluted peptides. (B) Immunofluorescence images of pachytene nuclei from wild type, syp-5S541A, and syp-6T685A mutants showing DNA, 3xFlag::SYP-5 or HA::SYP-6 (magenta), and SYP-5 pS541 (green) staining. Scale bar, 5 µm. Source data are available for this figure: SourceData FS1.

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