N-linked glycans on Na_V channels. (A) Alignments of the amino acid sequences of the Na_V1.1–1.9 domain I ECTLs are shown. The residues are colored by similarity (yellow), conservation among 50% of sequences or higher (green), or 100% conservation (orange letters and in green blocks). All NX[S or T] motifs in the sequence alignments are outlined with red rectangles to note the evolutionary conservation of the motifs. (B and C) The conserved glycans on the domain I ECTLs are colored crimson and shown on the modeled Na_V1.5 structure. In C, the unique Na_V1.5 glycans above VSD III are shown in crimson. (D and E) Alignments of the amino acid sequences of the Na_V1.1–1.9 domain II and III, respectively. Residue conservation and NX[S or T] motifs are colored as in A. (F) The unique Na_V1.8 N-linked glycan N819 is shown in crimson on the modeled Na_V1.8 structure. (G) The conserved glycans on domain III are colored in crimson and shown on the modelled Na_V1.5 structure.