Position and structural representation of Na_V glycans. (A) The N-linked glycan positions on Na_V channel cryo-EM structures are listed in a table format. Each color denotes homologous N-linked glycosylation sites within the indicated α-subunit isoform. (B) The representative N-linked glycan tree used in this study is shown in 2D format. (C) The corresponding 3D representation of the glycan tree is shown in B. N-acetylglucosamines are shown in blue, mannose in green, galactose in yellow, fucose in red, and sialic acid in pink. Connectivity (alpha = A, beta = B) between glycan moieties is notated (B).