The expression of X chromosome inactivation-related genes is correlated with ISG score in pDCs of SSc women. (A) Left: UMAP plot displaying the RNA expression of XIST at the single-cell level in the five SCs of pDCs described in Fig. 2. Right: Violin plot comparing XIST expression between HDs (n = 4) and SSc patients (n = 4). Statistical significance was analyzed using the Wilcoxon rank-sum test and indicated as ****P < 0.0001. (B and C) Violin plots illustrating the comparison of (B) SPEN, CBX4, and (C) ING4 expression between HDs and SSc patients are shown. Statistical significance was analyzed using the Wilcoxon rank-sum test and indicated as ****P < 0.0001. (D) Violin plot comparing XIST expression between ISG^low and ISG^high clusters. Statistical significance was analyzed using the Wilcoxon rank-sum test and indicated as ns when non-significant. (E) Left: Violin plot comparing SPEN expression between ISG^low and ISG^high clusters. Right: Correlation analysis between SPEN expression and ISG score. Statistical significance was analyzed using the Wilcoxon rank-sum test and indicated as *P < 0.05. (F) Left: Violin plot comparing CBX4 expression between ISG^low and ISG^high clusters. Right: Correlation analysis between CBX4 expression and ISG score. Statistical significance was analyzed using the Wilcoxon rank-sum test and indicated as ****P < 0.0001. The correlation between ISG score and the expression of the indicated gene was assessed in cells expressing the gene using Pearson correlation, and the corresponding P values were reported.