Figure 4.
Levels and distribution of full-length versus cleaved forms of TLR7 protein are altered in pDCs from SSc donors. (A) Representative western blot of sorted pDCs from SSc patients and HDs. Black arrowhead indicates 140 kDa full-length (FL) TLR7, while white arrowhead indicates the 75 kDa cleaved mature form (Cl). Actin is used as a loading control. (B and C) Densitometric analysis of TLR7 full-length and cleaved protein normalized to actin. (D) TLR7 proteins expressed as a ratio of TLR7 cleaved over full length from SSc patients (n = 8) and HDs (n = 8) pDCs. Statistical significance was analyzed using Mann–Whitney t test. *P < 0.05; ***P < 0.001. (E–H) PBMCs (n = 33) were incubated overnight with 1 ng/ml IFN-β and analyzed for TLR7 expression. (F and G) Densitometric analysis of TLR7 full-length and cleaved protein normalized to total protein. TLR7 proteins are expressed as a ratio of TLR7 cleaved over full length. (I–L) Sorted pDCs (n = 6) were incubated overnight with 1 ng/ml IFN-β or left untreated and analyzed for TLR7 expression. (I–K) Densitometric quantification of TLR7 full length and cleaved protein normalized to actin. (L) TLR7 proteins were expressed as a ratio of TLR7 cleaved over full length. (F–H and J–L) Statistical significance was analyzed using Wilcoxon paired t test; *P < 0.05, **P < 0.01, ****P < 0.0001. Source data are available for this figure: SourceData F4.

Levels and distribution of full-length versus cleaved forms of TLR7 protein are altered in pDCs from SSc donors. (A) Representative western blot of sorted pDCs from SSc patients and HDs. Black arrowhead indicates 140 kDa full-length (FL) TLR7, while white arrowhead indicates the 75 kDa cleaved mature form (Cl). Actin is used as a loading control. (B and C) Densitometric analysis of TLR7 full-length and cleaved protein normalized to actin. (D) TLR7 proteins expressed as a ratio of TLR7 cleaved over full length from SSc patients (n = 8) and HDs (n = 8) pDCs. Statistical significance was analyzed using Mann–Whitney t test. *P < 0.05; ***P < 0.001. (E–H) PBMCs (n = 33) were incubated overnight with 1 ng/ml IFN-β and analyzed for TLR7 expression. (F and G) Densitometric analysis of TLR7 full-length and cleaved protein normalized to total protein. TLR7 proteins are expressed as a ratio of TLR7 cleaved over full length. (I–L) Sorted pDCs (n = 6) were incubated overnight with 1 ng/ml IFN-β or left untreated and analyzed for TLR7 expression. (I–K) Densitometric quantification of TLR7 full length and cleaved protein normalized to actin. (L) TLR7 proteins were expressed as a ratio of TLR7 cleaved over full length. (F–H and J–L) Statistical significance was analyzed using Wilcoxon paired t test; *P < 0.05, **P < 0.01, ****P < 0.0001. Source data are available for this figure: SourceData F4.

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