CP lymphatic vessels and the perineural space of olfactory nerves can drain CSF after tMCAO. (A) Cartoon schematic of coronally sectioned OB/CP region. Note the cpLVs interface with both BV and ONB (created using https://BioRender.com). (B) After 7 days of tMCAO, 10% Evans blue dye was injected into the cisterna magna (30 min) to confirm CSF drainage through lymphatics near the CP. Representative confocal images show overlap of Evans blue dye and Lyve-1 which interfaces with BV. Scale bars = 50 µm. (C) The intensity profile plot shows co-localization of Evans blue dye within Lyve-1⁺ cpLVs. No dye was detected in the lumen of BV, only in the Lyve-1⁺ perivascular space. (D) Evans blue injected into the cisterna magna accumulates around ONB at the CP at day 7 of tMCAO. Confocal images show perineural accumulation after tMCAO. Scale bars = 10 µm. BV = blood vessels, ONB = olfactory nerve bundles. Source data are available for this figure: SourceData FS2.