tMCAO induces CSF accumulation at the CP lymphatics. (A and B) Representative T1-weighted MRI scan images of dorsal (A) and view of mice whole heads between sham and tMCAO 7 days after surgery. Baseline images were imaged before gadolinium injection and serial images over time were imaged after injection. (B) Zoomed representative image from 50-min time point shows increased signal intensity at CP region. (C) Quantitation of average pixel intensity normalized to baseline of the CP between sham and tMCAO mice (n = 3 mice for sham, n = 5 mice for tMCAO; mean ± SEM, *P ≤ 0.05, ***P ≤ 0.001, two-way ANOVA with repeated measures). Source data are available for this figure: SourceData F3.