Figure S1.
Lymphatic analysis of optic nerve, NP, and dura. (A) Coronal sections of optic nerve regions were stained with Lyve-1 fluorescent antibody in either control or tMCAO mice at days 3, 7, and 14. Representative confocal images of Lyve-1+ regions near the optic nerve. Quantitation of each image (n = 3–6 mice per group; mean ± SEM, two-way ANOVA). Scale bars = 200 µm. (B) Coronal sections of NP regions were stained with Lyve-1 fluorescent antibody in either control or tMCAO mice at days 3, 7, and 14. Representative confocal images of Lyve-1+ regions surrounding the NP. Quantitation of each image (n = 3–6 mice per group; mean ± SEM, two-way ANOVA). Scale bars = 200 µm. (C) COS of dural lymphatic vessels was stained with Lyve-1 antibody and imaged using confocal microscopy after sham and tMCAO at days 3, 7, and 14. Representative confocal images of COS. Scale bars = 500 µm. Quantitation of average Lyve-1+ vessel areas in the COS (n = 8 mice for sham, n = 9 mice for tMCAO; mean ± SEM, *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, two-way ANOVA). (D) Quantitation of number of loops, number of sprouts, and lymphatic vessel diameters of COS of dural lymphatic vessels after sham and tMCAO at days 3, 7, and 14 (n = 8 mice for sham, n = 9 mice for tMCAO; mean ± SEM, **P ≤ 0.01, ****P ≤ 0.0001, two-way ANOVA). (E) SSS of dural lymphatic vessels was immunolabeled with Lyve-1 fluorescent antibody and imaged after sham and tMCAO at days 3, 7, and 14. Representative confocal images of SSS. Quantitation of average Lyve-1+ vessel areas in the SSS (n = 8 mice for sham, n = 9 mice for tMCAO; mean ± SEM, *P ≤ 0.05, **P ≤ 0.01, ****P ≤ 0.0001, two-way ANOVA). Scale bars = 500 µm. (F) Quantitation of number of loops, number of sprouts, and lymphatic vessel diameters of SSS of dural lymphatic vessels after sham and tMCAO at days 3, 7, and 14 (n = 8 mice for sham, n = 9 mice for tMCAO; mean ± SEM, *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, two-way ANOVA). Source data are available for this figure: SourceData FS1.

Lymphatic analysis of optic nerve, NP, and dura. (A) Coronal sections of optic nerve regions were stained with Lyve-1 fluorescent antibody in either control or tMCAO mice at days 3, 7, and 14. Representative confocal images of Lyve-1+ regions near the optic nerve. Quantitation of each image (n = 3–6 mice per group; mean ± SEM, two-way ANOVA). Scale bars = 200 µm. (B) Coronal sections of NP regions were stained with Lyve-1 fluorescent antibody in either control or tMCAO mice at days 3, 7, and 14. Representative confocal images of Lyve-1+ regions surrounding the NP. Quantitation of each image (n = 3–6 mice per group; mean ± SEM, two-way ANOVA). Scale bars = 200 µm. (C) COS of dural lymphatic vessels was stained with Lyve-1 antibody and imaged using confocal microscopy after sham and tMCAO at days 3, 7, and 14. Representative confocal images of COS. Scale bars = 500 µm. Quantitation of average Lyve-1+ vessel areas in the COS (n = 8 mice for sham, n = 9 mice for tMCAO; mean ± SEM, *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, two-way ANOVA). (D) Quantitation of number of loops, number of sprouts, and lymphatic vessel diameters of COS of dural lymphatic vessels after sham and tMCAO at days 3, 7, and 14 (n = 8 mice for sham, n = 9 mice for tMCAO; mean ± SEM, **P ≤ 0.01, ****P ≤ 0.0001, two-way ANOVA). (E) SSS of dural lymphatic vessels was immunolabeled with Lyve-1 fluorescent antibody and imaged after sham and tMCAO at days 3, 7, and 14. Representative confocal images of SSS. Quantitation of average Lyve-1+ vessel areas in the SSS (n = 8 mice for sham, n = 9 mice for tMCAO; mean ± SEM, *P ≤ 0.05, **P ≤ 0.01, ****P ≤ 0.0001, two-way ANOVA). Scale bars = 500 µm. (F) Quantitation of number of loops, number of sprouts, and lymphatic vessel diameters of SSS of dural lymphatic vessels after sham and tMCAO at days 3, 7, and 14 (n = 8 mice for sham, n = 9 mice for tMCAO; mean ± SEM, *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, two-way ANOVA). Source data are available for this figure: SourceData FS1.

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