Effects of FKBP12 deficiency on 1-wk-old mice. (A) Protein expression of several RNA-seq detected transcripts was validated using western blot of hearts from (A) 1-wk-old mice. Proteins of interest were normalized to the total protein level in the same lane, normalized to the average expression level in FL hearts, and expressed as %FL. Data were analyzed by one-way ANOVAs with P value indicated. n = 6 for all genotypes. Data are shown as mean ± SD. (B) Oxyblot and analysis of oxidized proteins in hearts of 7-day-old mice of each genotype. (C) Iodine contrast microCT for cardiac phenotyping of P0 FL and MHC-FKD mice. Micro CT 3D imaging was performed on iodine contrasted FL (n = 5) and MHC-FKD (n = 8) neonates at birth (postnatal day 0, P0) to examine the gross morphology and phenotyping. 3D whole volume rendering and digitally sectioned at sagittal, coronal, and transverse axes and were a mixture of males and females (seven females, eight males). Sex differences were not detected. (D) Digital image showing cardiac structure of the right atrium (RA), left atrium (LA), right ventricular wall (RW), right ventricle (RV), ventricular septum (VS), left ventricle (LV), and left ventricular wall (LW) were examined. The thickness of the left/right ventricular walls and septum and the width of the left/right ventricles were measured along the dashed red line for each sample. (E and F) Quantitative analysis shows significant increases the (E) cross section of ventricles (P = 0.0045) and (F) LV Chamber width (P = 0.0478) in MHC-FKD neonates. (G and H) RV chamber (G), LV wall (H). (I and J) (I) RV wall and (J) ventricular septum (all datapoints provided) were not significantly different. Data were analyzed by one-way ANOVAs with P value indicated. Data are shown are mean ± SD. Source data are available for this figure: SourceData F9.
Figure 9.

Effects of FKBP12 deficiency on 1-wk-old mice. (A) Protein expression of several RNA-seq detected transcripts was validated using western blot of hearts from (A) 1-wk-old mice. Proteins of interest were normalized to the total protein level in the same lane, normalized to the average expression level in FL hearts, and expressed as %FL. Data were analyzed by one-way ANOVAs with P value indicated. n = 6 for all genotypes. Data are shown as mean ± SD. (B) Oxyblot and analysis of oxidized proteins in hearts of 7-day-old mice of each genotype. (C) Iodine contrast microCT for cardiac phenotyping of P0 FL and MHC-FKD mice. Micro CT 3D imaging was performed on iodine contrasted FL (n = 5) and MHC-FKD (n = 8) neonates at birth (postnatal day 0, P0) to examine the gross morphology and phenotyping. 3D whole volume rendering and digitally sectioned at sagittal, coronal, and transverse axes and were a mixture of males and females (seven females, eight males). Sex differences were not detected. (D) Digital image showing cardiac structure of the right atrium (RA), left atrium (LA), right ventricular wall (RW), right ventricle (RV), ventricular septum (VS), left ventricle (LV), and left ventricular wall (LW) were examined. The thickness of the left/right ventricular walls and septum and the width of the left/right ventricles were measured along the dashed red line for each sample. (E and F) Quantitative analysis shows significant increases the (E) cross section of ventricles (P = 0.0045) and (F) LV Chamber width (P = 0.0478) in MHC-FKD neonates. (G and H) RV chamber (G), LV wall (H). (I and J) (I) RV wall and (J) ventricular septum (all datapoints provided) were not significantly different. Data were analyzed by one-way ANOVAs with P value indicated. Data are shown are mean ± SD. Source data are available for this figure: SourceData F9.

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