Differential expression and pathway enrichment analysis of RNA-seq on 12-wk-old mouse whole-heart samples by genotype. (A–C) Volcano plots of the log₂ fold changes in gene expression and Benjamini-Hochberg adjusted P values for (A) MHC-FKD, (B) MCK-FKD, and (C) MHC-Cre⁺ versus FL hearts. Black: P_adj > 0.05 & abs(logFC) < 1; Orange: P_adj < 0.05 & abs(logFC) < 1; Blue: P_adj > 0.05 & abs(logFC) > 1; Red: P_adj < 0.05 and abs(logFC) > 1. Genes with adjusted P value <0.05 are labeled. (D) Venn diagrams of the overlap in significantly downregulated and upregulated genes (adjusted P value <0.05) in MHC-FKD, MCK-FKD, and MHC-Cre⁺ versus FL hearts. (E) Plot of the most significantly enriched IPA cardiotoxicity functions as determined by the Benjamini-Hochberg adjusted P values for the Fisher’s exact tests on the overlap between the set of 348 analysis-ready genes with unadjusted P value <0.05 (203 up and 145 down) and the set of genes in each toxicological function. The set of differentially expressed analysis-ready genes associated with each cardiotoxicity function is displayed on the corresponding bar. N = 6 mice per genotype.