Expression of ECC proteins. (A) 12-wk-old male hearts from FL, MCK-FKD, MHC-FKD and MHC-Cre mice were examined for (A) Ca_V1.2 expression. FL, n = 12, MCK-FKD, n = 12, MHC-Cre⁺, n = 8, MHC-FKD, n = 7. (B) Total RYR2 expression, FL, n = 16, MCK-FKD, n = 11, MHC-Cre⁺, n = 8, MHC-FKD, n = 13. (C) Phosphorylated RYR2 at S2807 FL, n = 8, MCK-FKD, n = 8, MHC-Cre⁺, n = 8, MHC-FKD, n = 6. (D) CASQ2 expression. FL, n = 16, MCK-FKD, n = 11, MHC-Cre⁺, n = 8, MHC-FKD, n = 14. (E) SERCA2a FL, n = 6, MCK-FKD, n = 6, MHC-Cre⁺, n = 6, MHC-FKD, n = 6. (F) Full-length JPH2 and its fragment expression FL, n = 21, MCK-FKD, n = 16, MHC-Cre⁺, n = 13, MHC-FKD, n = 18. (G) SPEG expression, FL, n = 17, MCK-FKD, n = 22, MHC-Cre⁺, n = 17, MHC-FKD, n = 20. (H) Oxyblot on cardiac homogenates from MHC-Cre⁺, FL, MCK-FKD, and MHC-FKD mice. (n = 6 for each group). Proteins of interest were normalized to the total protein level in the same lane, normalized to the average expression level in FL hearts, and expressed as %FL. Each band was analyzed. Data were analyzed by one-way ANOVAs. P values are indicated. Data are shown as mean ± SD. Source data are available for this figure: SourceData F5.