Inactivation of Stag2 and heterozygous inactivation of the cohesin subunit Smc3 uniquely increase lung tumor growth. (a) Schematic of the cohesin complex with subunits labeled. (b) Tumor initiation with a pool of Lenti-U6^BCsgRNA/Cre vectors. Genotype and number of mice are indicated. Tuba-seq^Ultra was performed on each tumor-bearing lung 15 wk after tumor initiation, followed by analyses to quantify tumorigenesis. (c) Relative mean tumor size (normalized to sgInert). Mean ± 95% confidence intervals are shown. (d) Relative tumor number (normalized to KT and sgInert). Mean ± 95% confidence intervals are shown. (e) Relative tumor number (normalized to KT and sgInert) versus relative mean tumor size (normalized to sgInert). (f) Tumor initiation with Adeno-Spc-Cre in KT and KT;Smc3^flox/+ mice. Mouse number is indicated. (g) Representative fluorescence and histology images of lung lobes from the indicated genotypes of mice. Top scale bars, 5 mm, and bottom scale bars, 1 mm. (h) Lung weights from KT and KT;Smc3^flox/+ mice. Each dot represents a mouse and the bar is the mean. Data are representative of two independent experiments. (i) Percentage of Tomato-positive tumor area detected via histology. Each dot represents a mouse, and the bar is the mean. *P value <0.05, **P value <0.01, via unpaired t test. Raw values and significance of each effect are shown in Table S1.