Figure S3.
Quantification of the abundance of a model protein, nucleolus volume, and FIB-1 abundance. (A) Quantification of total fluorescence intensity in intestinal nuclei from confocal images of day 2 adult worms expressing a single copy transgene, asp-1p::NLS-mScarlet. (B) Live confocal images of intestinal nucleoli of day 1 adult worms expressing endogenously-tagged Fibrillarin (wrmScarlet11::FIB-1) and ubiquitous eft-3p::wrmScarlet1-10, reconstituting fluorescently-tagged Fibrillarin only in the intestine. (C–E) Quantification of the total amount (C, total fluorescence intensity), concentration (D, mean fluorescence intensity) and volume (E), of FIB-1. In A and C–E, each dot represents one nucleus or nucleolus, bars and error bars represent means and 95% confidence intervals, and P values are from Mann-Whitney U tests after Benjamini-Hochberg multiple hypothesis testing correction.

Quantification of the abundance of a model protein, nucleolus volume, and FIB-1 abundance. (A) Quantification of total fluorescence intensity in intestinal nuclei from confocal images of day 2 adult worms expressing a single copy transgene, asp-1p::NLS-mScarlet. (B) Live confocal images of intestinal nucleoli of day 1 adult worms expressing endogenously-tagged Fibrillarin (wrmScarlet11::FIB-1) and ubiquitous eft-3p::wrmScarlet1-10, reconstituting fluorescently-tagged Fibrillarin only in the intestine. (C–E) Quantification of the total amount (C, total fluorescence intensity), concentration (D, mean fluorescence intensity) and volume (E), of FIB-1. In A and C–E, each dot represents one nucleus or nucleolus, bars and error bars represent means and 95% confidence intervals, and P values are from Mann-Whitney U tests after Benjamini-Hochberg multiple hypothesis testing correction.

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