Highly expressed mRNAs are more limited by DNA content. (A) Cartoon depicting two models of transcriptional compensation for limited DNA content. (B) Mock data depicting how each model in A predicts how transcript abundance relative to genome copy number would change with increasing transcript abundance. (C) Change in spike-in normalized gene expression (TxPG) plotted against and binned by expression level in control, each bin represents 5% of genes. (D) Cartoon depicting experimental design for intestine-specific ChIP of Pol II. (E) Change in Pol II occupancy at the transcription start site (TSS) plotted against and binned by expression level in control, each bin represents 5% of genes. Colored dots in C and E represent mean fold change within the bin and error bars represent a 99% confidence interval.