Extended data used in the calculation of intestinal cell volume. (A and B) Quantification of intestine length (A) and width (B) from images as in Fig. 1 H. Each point represents one worm; P values are from Mann-Whitney U tests. (C and D) To corroborate our organ size measurements, we measured the width of intestinal cells expressing a membrane marker since the width measurement was more sensitive to error in our measurements of bright-field images, such as Fig. 1 H. (C) Confocal images of day 2 adult control and CDK-2^gut(−) worms expressing elt-2p::GFP::CAAX (viridis coloring). (D) Quantification of intestine cell width (lumen to the basal surface) from images as in C, grouped by intestinal ring from anterior (int1) to posterior (int8). Each point represents one cell. P = 2.2 × 10⁻¹⁶ for the genotype coefficient in a linear model predicting cell size from genotype and intestinal ring. In all graphs, bars represent means and error bars represent 95% confidence intervals.