Figure 5.
Cell specific neuronal activity manipulation does not change Beat-VaMpruning. (A and B) Beat-Va neurons genetically labeled with mCD8::GFP and expressing UAS-LacZ at wL3 (A) and HE (B). (C and D) Beat-Va neurons genetically labeled with mCD8::GFP and constitutively expressing Kir2.1 under UAS control at wL3 (C) and HE (D). (E and F) Beat-Va neurons expressing LacZ under UAS control when at wL3 (E) and HE (F). The HE condition underwent metamorphosis at 29°C. (G and H) Beat-Va neurons expressing Trp1A, a temperature-sensitive cation channel, under UAS control at wL3 (G) and HE (H). The HE condition underwent metamorphosis at 29°C. (I and J) Beat-Va neurons expressing LacZ under UAS control at wL3 (I) and HE (J). All animals spent the 48 h prior to collection at 29°C. (K and L) Beat-Va neurons expressing Trp1A, under UAS control at wL3 (K) and HE (L). All animals spent the 48 h prior to collection at 29°C. All scale bars are 20 µm.

Cell specific neuronal activity manipulation does not change Beat-Va M pruning. (A and B) Beat-Va neurons genetically labeled with mCD8::GFP and expressing UAS-LacZ at wL3 (A) and HE (B). (C and D) Beat-Va neurons genetically labeled with mCD8::GFP and constitutively expressing Kir2.1 under UAS control at wL3 (C) and HE (D). (E and F) Beat-Va neurons expressing LacZ under UAS control when at wL3 (E) and HE (F). The HE condition underwent metamorphosis at 29°C. (G and H) Beat-Va neurons expressing Trp1A, a temperature-sensitive cation channel, under UAS control at wL3 (G) and HE (H). The HE condition underwent metamorphosis at 29°C. (I and J) Beat-Va neurons expressing LacZ under UAS control at wL3 (I) and HE (J). All animals spent the 48 h prior to collection at 29°C. (K and L) Beat-Va neurons expressing Trp1A, under UAS control at wL3 (K) and HE (L). All animals spent the 48 h prior to collection at 29°C. All scale bars are 20 µm.

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