Figure S2. Validation of genetic... | Rockefeller University Press

Figure S2.

$Validation of genetic interactors by flow cytometry. (A and B) Cytometry validation of DA array with (A) TPLH1-H5 or (B) TPL N188. (C and D) Cytometry validation of TS array with (C) TPLH1-H5 or (D) TPL N188. Selected strains were grown in liquid culture to log phase at 30°C and cytometry was performed. Fluorescence of tdTomato and Venus were quantified and Venus was normalized to tdTomato and is presented here as a ratio. Each data point is colored coded on a gradient with higher Venus expression as more yellow. The cutoff was arbitrarily set as the value of control (gray line) plus its standard error (red line). \ Error bars are standard error propagated through the calculation of the ratio (error=sqrt((((((Venus.Asd/(sqrt(events)))/Venus.Amedian)^2)+(((tdtomato3.Asd/(sqrt(events)))/tdtomato3.Amedian)^2))*abs(Ratio)))). The cutoff was arbitrarily set as the value of control (gray line) plus its standard error (red line). Refer to the image caption for details.$

Validation of genetic interactors by flow cytometry. (A and B) Cytometry validation of DA array with (A) TPLH1-H5 or (B) TPL N188. (C and D) Cytometry validation of TS array with (C) TPLH1-H5 or (D) TPL N188. Selected strains were grown in liquid culture to log phase at 30°C and cytometry was performed. Fluorescence of tdTomato and Venus were quantified and Venus was normalized to tdTomato and is presented here as a ratio. Each data point is colored coded on a gradient with higher Venus expression as more yellow. The cutoff was arbitrarily set as the value of control (gray line) plus its standard error (red line). \ Error bars are standard error propagated through the calculation of the ratio $(error = sqrt ((((((Venus . Asd / (sqrt (events))) / Venus . Amedian)^{^} 2) + (((tdtomato 3 . Asd / (sqrt (events))) / tdtomato 3 . Amedian)^{^} 2)) * abs (Ratio))))$ ⁠. The cutoff was arbitrarily set as the value of control (gray line) plus its standard error (red line).