Siglecf ^hi TAN removal impairs tumorigenesis. (A) Visualization of Vegfa expression among TILs. (B–E) Profiling of tumor cells from NRAS/AKT HCC mice after 2 wk of αLy6G or isotype control mAbs treatments. scRNA-seq expression of mature hepatocyte and hepatic progenitor markers (B). Representative FACs plots and percentage of Hoechst-effluxing cells (polygon gate) (C). Representative FACs plots and percentage of CD13⁺CD133⁺ CSCs (polygon gate) (D). Stem cell frequency in tumor cells isolated from NRAS/AKT HCC mice 1 wk following αLy6G or isotype control mAbs treatments (E). Each symbol represents one mouse (C and D). Data are mean ± SEM and represent two independent experiments (C–E). *P < 0.05, **P < 0.01 by unpaired Student’s t test (C and D) and Pearson’s chi-square test with a 95% confidence interval (E). MQs, macrophages; DCs, dendritic cells.