Characterization of murine models reveals MASH-HCC features in NRAS/AKT HCC and MCD + RIL175 models. (A–C) Representative H&E liver sections. The HCC tumor region is bounded by the dotted line. Scale bar represents 100 µm. (D) Untargeted lipidomic LC-MS analysis of the various types of HCC tumors and naïve liver. (E) Representative FACs plots and percentages of Ly6G⁺CD11b⁺ neutrophils (TANs) among CD45⁺TILs from cMYC/sgp53 tumors after 2 wk of αLy6G or isotype control mAbs treatment (top). Quantity of TANs per gram of tissue (bottom). (F and G) Gating strategies for tumor infiltrating lymphocytes (F): TANs (i), DCs (ii), CD8⁺T cells (iii) and their cytokines (iv), and tumor cells (G) from all the HCC models used. Each symbol represents one mouse. Data are mean ± SEM and represent two independent experiments (E). ****P < 0.0001 by unpaired Student’s t test (E). T, tumor; NT, non-tumor; TG, triglyceride; DG, diglyceride; PC, phosphatidylcholine; PE, phosphatidylethanolamine; SM, sphingomyelins.