DBR1 is crucial for PKR activation and antiviral responses in vivo. (A) Survival curve of WT (n = 8). and Dbr1Y17H/Y17H (n = 6) mice after intravenous inoculation with HSV-1 at a dose of 3.6 × 105 PFU/g body weight. Data representative of three independent experiments. Statistical analysis for mouse survival was performed with Kaplan–Meier tests. ***, P < 0.001. (B–D) WT and Dbr1Y17H/Y17H mice were sacrificed 3.5 days after intravenous inoculation with HSV-1 (3.6 × 105 PFU/g body weight). Brain tissues were collected, and RT-qPCR was performed to analyze the expression of viral genes (B) and the indicated ISR genes (C). Data representative of three independent experiments. Graphs depict the mean with SD and points represent biological replicates. Statistical analysis was performed with one-way ANOVA with Dunnett’s multiple comparisons test. **, P < 0.01; ***, P < 0.001; ****, P < 0.0001. WB with the indicated antibodies was used to assess protein levels (D). Data shown are representative of three independent experiments. (E) Survival curves of WT (n = 10) and Dbr1Y17H/Y17H (n = 12) mice after intravenous inoculation with VSV (106 PFU/g body weight). Data representative of three independent experiments. Statistical analysis for mouse survival was performed with Kaplan–Meier tests. ***, P < 0.001. (F and G) WT and Dbr1Y17H/Y17H mice were sacrificed 3.5 days after intravenous inoculation with VSV (106 PFU/g body weight). Brain tissues were collected, and RT-qPCR was performed to analyze the expression of viral genes (F) and the indicated ISR genes (G). Data representative of three independent experiments. Graphs depict the mean with SD and points represent biological replicates. Statistical analysis was performed with one-way ANOVA with Dunnett’s multiple comparisons test. *, P < 0.05; **, P < 0.01; ****, P < 0.0001. Source data are available for this figure: SourceData F8.
Figure 8.

DBR1 is crucial for PKR activation and antiviral responses in vivo. (A) Survival curve of WT (n = 8). and Dbr1Y17H/Y17H (n = 6) mice after intravenous inoculation with HSV-1 at a dose of 3.6 × 105 PFU/g body weight. Data representative of three independent experiments. Statistical analysis for mouse survival was performed with Kaplan–Meier tests. ***, P < 0.001. (B–D) WT and Dbr1Y17H/Y17H mice were sacrificed 3.5 days after intravenous inoculation with HSV-1 (3.6 × 105 PFU/g body weight). Brain tissues were collected, and RT-qPCR was performed to analyze the expression of viral genes (B) and the indicated ISR genes (C). Data representative of three independent experiments. Graphs depict the mean with SD and points represent biological replicates. Statistical analysis was performed with one-way ANOVA with Dunnett’s multiple comparisons test. **, P < 0.01; ***, P < 0.001; ****, P < 0.0001. WB with the indicated antibodies was used to assess protein levels (D). Data shown are representative of three independent experiments. (E) Survival curves of WT (n = 10) and Dbr1Y17H/Y17H (n = 12) mice after intravenous inoculation with VSV (106 PFU/g body weight). Data representative of three independent experiments. Statistical analysis for mouse survival was performed with Kaplan–Meier tests. ***, P < 0.001. (F and G) WT and Dbr1Y17H/Y17H mice were sacrificed 3.5 days after intravenous inoculation with VSV (106 PFU/g body weight). Brain tissues were collected, and RT-qPCR was performed to analyze the expression of viral genes (F) and the indicated ISR genes (G). Data representative of three independent experiments. Graphs depict the mean with SD and points represent biological replicates. Statistical analysis was performed with one-way ANOVA with Dunnett’s multiple comparisons test. *, P < 0.05; **, P < 0.01; ****, P < 0.0001. Source data are available for this figure: SourceData F8.

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