Large ribosomal protein depletion does not affect CB composition but leads to an altered dimethylarginine profile. (A and B) Immunofluorescent images showing the presence of (A) nopp140 and (B) dyskerin (cyan) in CBs (α-coilin antibody in magenta) in control or ribosomal protein knockdown cells. (C) Immunofluorescent images showing the presence of snRNPs (α-Sm, Y12 antibody in cyan) in CBs (α-coilin antibody in magenta). Scale bars = 10 µm. (D) Representative single nucleus immunofluorescent staining of coilin (magenta) and SMN (cyan) in siNT and siRPL14 with accompanying line profile plots showing intensities through a single CB. (E) Immunofluorescent images showing asymmetric dimethylarginine (aDMA) signal in HeLa cell after respective siRNA knockdown. CB outline is shown in magenta. Scale bars = 5 μm. (F and G) Quantification of aDMA intensity in the nucleus or CB in HeLa cells after siRNA knockdown of control or ribosomal proteins for 72 h. The relative DMA intensity is calculated by dividing mean DMA intensity in each nucleus (F) or CB (G) to the respective mean coilin intensity. The median of the relative DMA intensity is shown by the magenta (F) or cyan (G) line. Number of replicates = 4. P values are obtained by Kruskal–Wallis test followed by Dunn test with Bonferroni correction. **, P < 0.01; ****, P < 0.0001.