Large ribosomal protein depletion leads to increased CB number, impaired ribosomal biogenesis and translation inhibition. (A) Immunofluorescent images showing coilin signal in HeLa cells after siRNA knockdown of control or ribosomal proteins for 72 h. The nucleus outline is shown in blue. (B) Electropherograms of total RNA isolated from HeLa cells after respective siRNA knockdown. 28S/18S rRNA intensity ratio is listed below each lane, and a representative electropherogram in arbitrary intensity unit is shown for each siRNA. (*) marks sample reruns. (C) WB of HeLa cell lysate harvested after respective siRNA knockdown. Tubulin was used as the loading control. Numbers 1–3 stand for independent replicates. (D) WB of puromycin incorporation assay. HeLa cell was treated with the siRNA of control or ribosomal proteins for 72 h, followed by incubation with or without 1 μM puromycin for 1 h before harvest. “−” indicates no puromycin control, “+” indicates puromycin addition. Ponceau stain was shown as the loading control. Two replicates are shown for each RPL knockdown condition. Source data are available for this figure: SourceData FS4.