Ribosomal subunit proteins do not localize to CBs or interact with coilin. (A) Left: Immunofluorescent staining of wildtype HeLa cell using RPL24 (cyan) and coilin (magenta) antibody. Scale bar = 10 μm. Right: colocalization analysis of RPL24 and coilin. Scale bars = 1 μm. (B) Coimmunoprecipitation using GFP antibody with RPL13, RPL14, or RPS2 cell line. Stable HeLa cell lines expressing fluorescently tagged ribosomal proteins were induced by 0.5 μg/ml doxycycline for 20 h before harvest. Cell lysate was incubated with IgG or GFP antibody overnight. GAPDH was used as negative control. 2.2% of sample were loaded as input. (*) indicates IgG heavy chain, which is below fluorescently tagged RPL13 and RPS2 and above RPL14. (C) Coimmunoprecipitation using RPL24 or Lsm8 antibody in wildtype HeLa cells. HeLa cell lysate was incubated with IgG, RPL24, or Lsm8 antibody overnight. GAPDH was used as negative control. 2% of sample were loaded as input. Two replicates are shown for each IP. Source data are available for this figure: SourceData FS3.