Ribosomal subunit proteins do not localize to CBs. (A) Immunofluorescent images of stable HeLa cell lines expressing fluorescently tagged ribosomal proteins. Ribosomal protein expression is induced by 0.5 µg/ml doxycycline for 16 h before fixing the cell for immunostaining with coilin (magenta). GFP or YFP fluorescence is shown in cyan. RPL13 and RPL14 are hits found to increase CB number, RPS2 is enriched in proximity biotinylation but was found to not affect CB number. The nucleus outline is shown in blue (white background) or white (dark background) lines. Scale bars = 10 µm. (B) Colocalization analysis of coilin with the ribosomal proteins. The total number of CBs analyzed (n) and the Pearson’s correlation coefficient (PCC) are listed on the right. Scale bars = 0.5 µm.