Figure 3.
siRNA screen of CB components identifies 34 proteins that change CB number in HeLa cells. (A) Schematic of microscopy-based CB-specific siRNA screen. (B) Frequency histogram of percent effect per siRNA in screen. Values of siRNAs that decreased the number of CBs per nucleus are highlighted in yellow (PE > 60). Values of siRNAs that increased the number of CBs per nucleus are highlighted in orange (PE < −60). (C and D) Representative images of wells from siRNA screen. Immunofluorescent staining shows coilin (magenta) and nucleophosmin (cyan). Histograms for each siRNA condition quantifying the number of CBs per nucleus from their respective well and plate in the screen (12 fields of view/well). (C) Representative image of control conditions (siNT and siCoilin). (D) Representative image of hits showing a decrease in CBs per nucleus (siSPT6 and siTRIM22). Representative hit shown in teal with siNT (non-targeting siRNA) in light gray. Scale bars = 10 µm.

siRNA screen of CB components identifies 34 proteins that change CB number in HeLa cells. (A) Schematic of microscopy-based CB-specific siRNA screen. (B) Frequency histogram of percent effect per siRNA in screen. Values of siRNAs that decreased the number of CBs per nucleus are highlighted in yellow (PE > 60). Values of siRNAs that increased the number of CBs per nucleus are highlighted in orange (PE < −60). (C and D) Representative images of wells from siRNA screen. Immunofluorescent staining shows coilin (magenta) and nucleophosmin (cyan). Histograms for each siRNA condition quantifying the number of CBs per nucleus from their respective well and plate in the screen (12 fields of view/well). (C) Representative image of control conditions (siNT and siCoilin). (D) Representative image of hits showing a decrease in CBs per nucleus (siSPT6 and siTRIM22). Representative hit shown in teal with siNT (non-targeting siRNA) in light gray. Scale bars = 10 µm.

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